What is the Kirby Bauer technique?

The Kirby-Bauer test, known as the disk-diffusion method, is the most widely used antibiotic susceptibility test in determining what choice of antibiotics should be used when treating an infection. This method relies on the inhibition of bacterial growth measured under standard conditions. What is the kissing game? .

What is the Kirby Bauer zone of inhibition?

A Zone of Inhibition Test, also called a Kirby-Bauer Test, is a qualitative method used clinically to measure antibiotic resistance and industrially to test the ability of solids and textiles to inhibit microbial growth.

What information does the Kirby Bauer method provide quizlet?

What is the Kirby-Bauer Test used for? Uses antibiotic-containing discs to measure the sensitivity of bacteria to antibiotics by culturing bacteria on a plate and placing the soaked discs on the bacteria. Zone of inhibition is then measured to calculate the sensitivity or resistance of the bacteria.

What is the purpose of the Kirby Bauer test?

The purpose of the Kirby-Bauer disk diffusion susceptibility test is to determine the sensitivity or resistance of pathogenic aerobic and facultative anaerobic bacteria to various antimicrobial compounds in order to assist a physician in selecting treatment options for his or her patients.

What are the advantages of using the Kirby-Bauer method?

The microbroth dilution method has become more widely used due to its ability to become automated, however, the Kirby Bauer method may be able to detect subpopulations of resistant bacteria that would be visualized as inner colonies in the zone of diffusion.

Why is the Kirby-Bauer test performed quizlet?

The Kirby-Bauer (disk diffusion) test is a valuable standard tool for measuring the effectiveness of antimicrobics against pathogenic microorganisms. In the test, antimicrobic-imprdegnated paper disks are placed on a plate that is inoculated to form a bacterial lawn.

What type of medium is used in the Kirby-Bauer method?

The standard medium for the Kirby-Bauer method of susceptibility testing is Mueller-Hinton agar (MHA) [6]. Because of the number of difficulties and financial issues, MHA is not a feasible option in many developing countries, and instead, NA is used for AST [7].

Why is it called the zone of inhibition?

If an antibiotic stops the bacteria from growing or kills the bacteria, there will be an area around the disk where the bacteria have not grown enough to be visible. This is called a zone of inhibition.

What tool is usually used for inoculating the agar when creating a bacterial lawn?

What tool is usually used for inoculating the agar when creating a bacterial lawn? A sterile swab is usually used to spread a bacterial culture over the entire surface of the agar plate. You just studied 28 terms!

What tool is usually used for inoculating the agar when creating a bacterial lawn quizlet?

How are agar plates inoculated for disk diffusion testing of antiseptics and disinfectants? A swab is used to inoculate the entire surface of the plate, producing a lawn of growth. The clear area devoid of bacterial growth around an antiseptic or disinfectant disk is referred to as the: zone of inhibition.

What does a low MIC mean?

Minimum inhibitory concentration (MIC) can be determined by culturing microorganisms in liquid media or on plates of solid growth medium. A lower MIC value indicates that less drug is required for inhibiting growth of the organism; therefore, drugs with lower MIC scores are more effective antimicrobial agents.

What is measured during the Kirby-Bauer antimicrobial susceptibility test?

Kirby-Bauer Disk Susceptibility Test Kirby-Bauer testing measures sensitivity of bacteria to antibiotics by culturing bacteria on solid growth media surrounding sources of drug.

What is measured during the Kirby-Bauer antimicrobial susceptibility test quizlet?

Terms in this set (20) Antimicrobial susceptibility test, aka used for measuring ht effectiveness ofantimicrobials against pathogenetic microorganisms. antimicrobial-impregnated paper disks are placed on plate inoculated to form a bacterial lawn.

What is the purpose of testing a known antibiotic?

Testing is used to determine the potential effectiveness of specific antibiotics on the bacteria and/or to determine if the bacteria have developed resistance to certain antibiotics. The results of this test can be used to help select the drug(s) that will likely be most effective in treating an infection.

What factors must be carefully controlled in Kirby-Bauer method?

• Size of inoculum.
• Distribution of the inoculum.
• Incubation period.
• Growth rate of bacterium.
• Depth of agar.
• Diffusion rate of the antibiotic.
• Concentration of antibiotic in the disk.

What is MIC of antibiotic?

Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight incubation, and minimum bactericidal concentrations (MBCs) as the lowest concentration of antimicrobial that will prevent the growth of an organism …

Which tool of the microbiologist was used to make a lawn of microorganisms on the surface of a bacteriological media?

Agar streak plates are an essential tool in microbiology. They allow bacteria and fungi to grow on a semi-solid surface to produce discrete colonies. These colonies can be used to help identify the organism, purify the strain free of contaminants, and produce a pure genetic clone.

What is pour plate technique?

Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. Because the sample is mixed with the molten agar medium, a larger volume can be used than with the spread plate. … Each colony represents a “colony-forming unit” (CFU).

What is an inoculating loop used for?

Inoculating loops are used to introduce microorganisms into a culture medium.

What describes a method used to create a bacterial lawn in this experiment?

Bacterial lawns can be produced manually by evenly spreading a high amount of bacteria onto an agar plate using a sterile cotton swab or a Drigalski spatula. Alternatively an automated machine can be used such as a spiral plater where the plate is rotated and the sample is spread evenly using an automated dispenser.

How does the antibiotic get from the disc into the agar?

How does the antibiotic get from the disk into the agar? The antibiotic diffuses out of the disk and into the agar. This diffusion can be affected by temperature and depth of agar in the plate.

How does antibiotic get from the disk into the agar?

When testing a conventional antibiotic, this involves creating a lawn of bacteria on an agar plate and then placing an antibiotic disc or discs onto the lawn before incubation it overnight. This method works because the antibiotic in the disc diffuses out into the agar, creating a concentration gradient.

What is vancomycin MIC?

Vancomycin susceptibility is currently defined as an MIC ≤2mcg/mL, vancomycin intermediate S. aureus (VISA) as an MIC equal to 4 to 8 mcg/mL, and vancomycin resistant S. aureus (VRSA) as an MIC ≥16mcg/mL [13].

What drug is a fluoroquinolone?

FDA-approved fluoroquinolones include levofloxacin (Levaquin), ciprofloxacin (Cipro), ciprofloxacin extended-release tablets, moxifloxacin (Avelox), ofloxacin, gemifloxacin (Factive) and delafloxacin (Baxdela).

What is the difference between MIC and breakpoint?

A breakpoint is a chosen concentration (mg/L) of an antibiotic which defines whether a species of bacteria is susceptible or resistant to the antibiotic. … If the MIC is greater than this value the bacteria is considered intermediate or resistant to the antibiotic.

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